expression profile of developmentally important genes in pre- and peri-implantation goat embryos produced in vitro

Expression profile of developmentally important genes in pre- and peri-implantation goat embryos produced in vitro

Background Little is understood about the regulation of gene expression during early goat embryo development. This study investigated the expression profile of 19 genes, known to be critical for early embryo development in mouse and human, at five different stages of goat in vitro embryo development (oocyte, 8-16 cell, morula, day-7 blastocyst, and day 14 blastocyst). MaterialsAndMethods Stage-...

Expression Profile of Developmentally Important Genes in preand peri-Implantation Goat Embryos Produced In Vitro

BACKGROUND Little is understood about the regulation of gene expression during early goat embryo development. This study investigated the expression profile of 19 genes, known to be critical for early embryo development in mouse and human, at five different stages of goat in vitro embryo development (oocyte, 8-16 cell, morula, day-7 blastocyst, and day 14 blastocyst). MATERIALS AND METHODS In...

P-47: Effect of Blastocoelic Fluid Reduction on Quality and Expression of Developmentally Important Genes in Mouse Blastocysts

Background Recent researches reveal that manual puncturing of the trophectoderm of blastocyst before vitrification, increase the quality of embryo. However, in any of these studies, the importance of blastocoelic fluid and its impact on the formation of three cell lines is not mentioned. Therefore, in the present study, the effect of blastocoelic fluid reduction before vitrification on survival...

Melatonin upregulates ErbB1 and ErbB4, two primary implantation receptors, in pre-implantation mouse embryos

Objective(s): To evaluated the effects of melatonin on early embryo competence and the expression rate of the primary implantation receptors (ErbB1 and ErbB4). Materials and Methods: Two-cell mouse embryos were cultured in 3 groups: simple media, melatonin-treated (10-9 M melatonin) and Luzindole-treated (10-9 M luzindole). Then, the rate of ErbB1 and ErbB4 gene and protein expression, the leve...

Stage-specifi c developmental gene expression of goat preimplantation embryos produced in vitro

Single preimplantation embryo mRNA diff erential display was used to study the gene expression of 2-cell,4cell, and 8-16-cell stage goat embryos produced in vitro, and 16 diff erent stage specifi c bands in total were screened. In these specifi c bands, 5 were in 2-cell stage, 4 were in 4-cell stage, and 7 were in 8-16-cell stage, respectively. Th e sequencing and alignment results suggested th...

Expression pattern of developmentally relevant genes in cattle preimplantation embryos